Journal: BMC Musculoskeletal Disorders

Article Title: High molecular weight hyaluronic acid alleviates ovariectomy-induced bone loss in mice

doi: 10.1186/s12891-024-08161-y

Figure Lengend Snippet: HMW-HA downregulated osteoclastic bone resorption in ovariectomized mice through the downregulation of RANKL-expressing osteocytes. A The area analyzed for osteoclast parameters is indicated in the box in yellow. B Representative images of TRAP staining. Low-power images boxed in red are enlarged and presented in each panel. Black arrows indicate osteoclasts. Red bars in high-power images indicate scale bars (50 μm). C Bone resorption parameters including osteoclast surface per bone surface (Oc.S/BS) and osteoclast number per bone surface (N.Oc/BS) of each sagittal section. D The area analyzed for Rankl + osteocyte percentage is indicated in the box in green. E Representative images of RANKL-positive osteocyte lacunes. Low-power images boxed in red are enlarged and presented in each panel. Black arrows indicate RANKL-positive osteocyte lacunes. Red arrows indicate osteocytes with no RANKL expression. Red bars in high-power images indicate scale bars (50 μm). F The Rankl. + osteocyte percentage was qualitatively assessed in each group. Data are expressed as mean ± SD, analyzed by ANOVA followed by Tukey’s post hoc test, showing all data points. * p < 0.05, ** p < 0.01, *** p < 0.001. The number of samples are indicated at the bottom of each figure and in Supplementary Tables 3 and 4

Article Snippet: Rabbit anti-mouse RANKL polyclonal antibody (cat#GTX32834) was purchased from Funakoshi (Tokyo, Japan).

Techniques: Expressing, Staining

Journal: BMC Musculoskeletal Disorders

Article Title: High molecular weight hyaluronic acid alleviates ovariectomy-induced bone loss in mice

doi: 10.1186/s12891-024-08161-y

Figure Lengend Snippet: Removal of HMW-HA from estrogen-deficient culture medium increased the RANKL expression in ST2 cells. A Experimental plan. Three hundred thousand cells were incubated for 12 h in RPMI-1640 medium. After cells were attached to the dishes, culture media were replaced with (I) charcoal-stripped FBS and (II) charcoal-stripped FBS with 100 nM of hyaluronidase for 12 h or 24 h. B , C Relative expression of RANKL and OPG in the presence or absence of hyaluronidase at 12 and 24 h. Colorful lines are associated with each 12 and 24 h samples. D Average ratio of RANKL mRNA to OPG mRNA in the presence or absence of hyaluronidase. Data are expressed as mean ± SD, analyzed by Wilcoxon signed-rank test ( B , C ), Wilcoxon rank-sum test ( D ), showing all data points. * p < 0.05. ns, not significant. n = 6 per group. Numerical data are presented in Supplementary Table 6

Article Snippet: Rabbit anti-mouse RANKL polyclonal antibody (cat#GTX32834) was purchased from Funakoshi (Tokyo, Japan).

Techniques: Expressing, Incubation

Journal: The Saudi Dental Journal

Article Title: Influence of platelet-rich plasma on RANKL and IL-1 immunohistochemical expression in periodontitis-related bone cell proliferation and differentiation

doi: 10.1016/j.sdentj.2024.11.011

Figure Lengend Snippet: Immunohistochemical section periodontal tissue of cytoplasmic expression of IL-1β and RANKL among different studied groups showed; a: No positive stain (score = 0) in the control negative group. b: Strong diffuse positive staining (score = 12) in the control positive group. c: Moderate staining in endothelium only (score = 2) in the PRP group. d: Moderate positive cells in both inflammatory and endothelium (score = 4) in the control positive group. e: No staining (score = 0) in the PRP group. f: The score degree for IL-1β expression in the studied groups. g: No positive stain (score = 0) in the control negative group. h: Strong diffuse positive staining (score = 12) in the control positive group. i: Moderate staining in osteoclast only (score = 4) in the PRP group. j: Moderate positive cells in both osteocyte and osteoclast (score = 4) in the control positive group. k: Weak-focal staining (score = 2) in the PRP group. l: The score degree for RANKL expression in the studied groups.

Article Snippet: The sections were incubated overnight with primary antibodies; IL-1β rabbit polyclonal antibody (1:600; Biorbyt, US) and RANKL rabbit polyclonal antibody (1:100; Biorbyt, US).

Techniques: Immunohistochemical staining, Expressing, Staining, Control

Journal: Cell Reports Medicine

Article Title: Denosumab stimulates spermatogenesis in infertile men with preserved Sertoli cell capacity

doi: 10.1016/j.xcrm.2024.101783

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-RANKL , Santa Cruz Biotech, US , Cat#sc-9073; RRID: AB_2303585.

Techniques: Polymer, Plasmid Preparation, Recombinant, Ex Vivo, Labeling, Enzyme-linked Immunosorbent Assay, Software, Microscopy, Cytometry, Fluorescence, FACS, Flow Cytometry